Fig. 3: TGFβ priming reprograms the action of TNF on the transcriptome in macrophages from inflammatory towards osteoclastic gene expression. | Nature Communications

Fig. 3: TGFβ priming reprograms the action of TNF on the transcriptome in macrophages from inflammatory towards osteoclastic gene expression.

From: TGFβ reprograms TNF stimulation of macrophages towards a non-canonical pathway driving inflammatory osteoclastogenesis

Fig. 3

a–h RNA-seq analysis of the mRNAs from human CD14(+) monocytes treated with or without TGFβ priming for 3 days, followed by TNF stimulation for 1 or 6 days. n = 3 biological replicates for each condition. a Pathway analysis of TNF-inducible genes at 24 h in the non-priming condition. b Pathway analysis of TNF-inducible genes at day 6 in the TGFβ priming condition. c–e Heatmaps of TNF-induced type-I IFN response genes (c), chemokine genes (d), and osteoclast genes (e) regulated by non-priming and TGFβ priming at the indicated times. Row z scores of CPMs were shown in the heatmaps. f–h Gene set enrichment analysis of TNF-inducible type-I IFN response genes (f), chemokine genes (g), and osteoclast genes (h) regulated by non-priming and TGFβ priming ranked by NES. i–j qPCR analysis of mRNA expression of IFIT1, IFIT2, MX1, STAT1, CCL5, CXCL9 and CXCL10 using human CD14(+) monocytes treated with or without TGFβ priming for 3 days, followed by TNF for 1 day (n = 5/group). i, j ***p < 0.001 by two-way ANOVA with Bonferroni’s multiple comparisons test. Error bars: i, j Data are mean ± SD. Source data are provided as a Source Data file.

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