Fig. 3: Hyal-responsive drug release of HCM NPs. | Nature Communications

Fig. 3: Hyal-responsive drug release of HCM NPs.

From: Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections

Fig. 3

a Scheme of the decomposition of HCM NPs in presence of Hyal. Fluorescence images (b) and corresponding fluorescence intensity (c) of HCM NPs (Ce6: 40 μg/mL) after incubation with Hyal at different concentrations for 12 h (n = 3 independent samples; mean ± SD). Release of Ce6 (d) and MNZ (e) from HCM NPs (Ce6: 40 μg/mL; MNZ: 20 μg/mL) under different conditions (n = 3 independent samples; mean ± SD). The confocal laser scanning microscopy (CLSM) images of MRSA biofilms (f) and SMCs (g) after incubation with HCM NPs (Ce6: 50 μg/mL; MNZ: 25 μg/mL) for different times. Scale bar is 200 μm. Hydrodynamic size (h) and TEM images (i) of HCM NPs after incubation with Hyal (100 unit/mL) for 24 h. j Relative absorbance of ABDA at 380 nm (OD380) after incubation at various conditions under laser irradiation (635 nm, 20 mW/cm2) for different times. Three independent experiments were performed and representative results are shown in f, g, and i. Source data are provided as a Source Data file.

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