Fig. 4: PD-1 blockade in Suv39h1-KO CD8+TILs induces a transcriptomic effector phenotype also found in responder melanoma patients.
From: CD8+T cell responsiveness to anti-PD-1 is epigenetically regulated by Suv39h1 in melanomas
A UMAP plot of 4097 cells for each of the four analyzed conditions after downsampling (on the top) and the corresponding density plots (on the bottom). B Heatmap of proportion and numeric value of the downsampled 4097 cells for each of the four conditions within the eight clusters. C Feature plots of human melanoma CD8+ TILs showing responder and not responder signature score from scRNAseq published dataset32. D Feature plots of human melanoma CD8+ TILs showing distribution of signature scores from littermate WT and Suv39h1-KO treated or not with anti-PD-1 dataset conditions. E Correlation between transcriptomic signatures from Suv39h1-KO mice treated with anti-PD-1 and the responder and not responder melanoma patients32 calculated with Pearson correlation coefficient. F Feature plots of murine CD8+ TILs (Fig. 3B) highlighting distribution of signature scores of littermate WT and Suv39h1-KO treated or not with anti-PD-1 dataset conditions. G Enrichment of the transcriptomic signature of genes upregulated in published melanoma ICB responders projected in murine CD8+ TILs UMAP (Fig. 3B). See also Supplementary Fig. 5. Source data are provided as a Source Data file.
