Fig. 1: Design of SURRO-seq.
From: Massively targeted evaluation of therapeutic CRISPR off-targets in cells
An overview of the nine major steps of SURRO-seq is schematically presented. MM, mismatches; ON, on-target; OT, off-targets; PBS, primer binding sites; BB, BsmBI binding site; Sp, spacer; Scr, SpCas9 gRNA scaffold; BC, barcodes; PS, protospacer; PAM, protospacer adjacent motif; 4r, 4 bp downstream sequences; GGA, Golden Gate Assembly; MOI, multiplexity of infection; WT, wildtype; Results presented in Step 9 are indel frequencies captured by SURRO-seq for RGN VEGFA T3.
