Fig. 2: Cardiac loss of SDHAF4 shortens mouse lifespan due to cardiac failure. | Nature Communications

Fig. 2: Cardiac loss of SDHAF4 shortens mouse lifespan due to cardiac failure.

From: Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy

Fig. 2

a Postnatal survival curve for WT (Sdhaf4fl/fl, n = 15) and Sdhaf4fl/fl, Ckmm-Cre mice (male, n = 20; female, n = 11). b–f Heart function measurements of WT, heterozygous (Sdhaf4fl/-, Ckmm-Cre), and homozygous (Sdhaf4fl/fl, Ckmm-Cre) mutant mice: representative M-mode echocardiography (b), left ventricular mass (LV mass, c, P < 0.0001), corrected LV mass (d, P < 0.0001), percentage of ejection fraction (EF, e, P < 0.0001), percentage of fractional shortening (FS, f, P < 0.0001), n = 8 for male, n = 4 for female. g mRNA levels of Sdhaf4 in tissues of WT (Sdhaf4fl/fl, Mer-CreMer, n = 3, P < 0.0001) and mutant mice (Sdhaf4fl/fl, Mer-CreMer after tamoxifen treatment for 8 weeks, n = 3). h Postnatal survival curve for WT (n = 15) and cardiac Sdhaf4 mutant mice (male, n = 21; female, n = 18). i Heart images of WT and cardiac KO mice, scale bar, 1 cm. Values are mean ± SEM, *P < 0.05, **P < 0.01. Log-rank Mantel-Cox testing was performed for survival analysis (a, h), other statistical significance was determined by two-tailed Student’s t-test (c–g). Source data are provided in Source Data file.

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