Fig. 5: Loss of SDHAF4 exacerbates TCA cycle-associated metabolic capacity. | Nature Communications

Fig. 5: Loss of SDHAF4 exacerbates TCA cycle-associated metabolic capacity.

From: Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy

Fig. 5

a, b Immunoblots for TCA cycle enzymes in left ventricles of hearts from WT and Sdhaf4 mutant mice at age of 3 and 8 weeks respectively, representative blotting images (a), summary analysis of arbitrary unit (b), n = 6, P < 0.0001. c Quantitative measurements of TCA cycle metabolites pyruvate, malate, fumarate, succinate, citrate, isocitrate, and alpha-ketoglutarate (n = 4, P = 0.012, 0.001, 0.0013, 0.009, 0.003). Box plots indicate median (middle line), 25th, 75th percentile (box), and 5th and 95th percentile (whiskers). d–f Metabolomics analysis for left ventricles of WT and Sdhaf4 mutant mice at age of 8 weeks, with Z-score plot of global metabolic profiles among all the samples (d), metabolic pathway enrichment analysis (e), and representative images of alterations on arginine biosynthesis pathway (f, green arrows for TCA cycle; blue arrows for arginine biosynthesis pathway; red highlights decreased metabolites), n = 7. Values are mean ± SEM, *P < 0.05, **P < 0.01. Statistical significance was determined by two-tailed Student’s t-test (b, c). Source data are provided in Source Data file. TCA cycle tricarboxylic acid cycle.

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