Fig. 1: Female Rett mice display social deficits in social interaction test.

a Left: the apparatus for the social interaction test. The subject mouse is in the middle 40 cm-long chamber; the 10 cm end compartments contain different stimuli. Right: the three sessions of the social interaction test. In the first 10 minute test session (S1), a strange mouse (M1) and an object (O) were placed in the end chambers; session 2 (S2) used the same stimuli but swapped their positions; in session 3 (S3) a new mouse (M2) replaced O, so that the subject mouse must choose whether to interact with a familiar or strange mouse (M1 vs. M2). “Sniff” means the mouse was at the end of the chamber and interacting with a stimulus; social zone (SZ), object zone (OZ), and new social zone (NSZ) indicate the 10 cm regions of the central chamber that are nearest to the end chambers of M1, O, and M2, respectively; transition zone (TZ) means the middle area in the central chamber. b Raster plots of the percentage of time mice spent in interacting with different stimuli in 3–10 minute sessions. The time is divided into 20 second bins; the color ranges from deep red (0%) to yellow (100%). c Left: the percentage of time that WT (Mecp2^+/+; n = 12) and Rett (Mecp2^+/−; n = 11) mice spent interacting with stimuli in each session. Right: Discrimination Iindices of WT and Rett mice indicating the difference in the amount of time the mice spent with each stimulus in a given session (M1 vs. O in S1 and S2; M2 vs. M1 in S3), relative to the total duration of stimulus interactions out of the whole session. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-way RM ANOVA with Bonferroni–corrected post hoc comparisons. Source data are provided as a Source Data file.