Fig. 4: IMs are the TAMs that respond to IL-9.

a–d LLC cells were directly injected to the lung. Tumor appearance (a) and tumor incidence (b) (n = 2  experiments) were assessed after resection. Tumor weight (c) (n = 8 mice for WT group, n = 10 mice for Il9r^−/− group) was calculated from two independent experiments. d Lung cells from tumor bearing mice and cells from tumor foci were isolated for flow analysis (n = 5 mice for lung Mac group, n = 6 mice for TAM group). TAM: tumor associated macrophage. e Venn graph showing the overlap genes from human TAMs and mouse IMs. f–h WT (CD45.1) and Il9r^−/− mice (CD45.2) bone marrow cells were mixed in 1:1 ratio and transferred to lethally irradiated recipient mice (CD45.1⁺ CD45.2⁺ mice). After reconstitution, chimeric mice were intravenously injected with B16 tumor cells, donor lung macrophages were analyzed by flow cytometry (n = 9 mice). i–k Il9r^−/− mice were injected with B16 tumor on day 0. Fluorescent bead-labeled monocytes were transferred to recipient mice on day 10 (i). Tumor growth (j) and lung macrophages (k) were analyzed on day 18, dot plots were gated on MerTK⁺ CD64⁺ SiglecF⁻ live cells (n = 4 mice per group). Data are the mean ± SEM. Unpaired two-tailed Student t-test was used for comparison in c, d and j. Two-way ANOVA with Sidak’s multiple comparisons was used for comparisons in h.