Fig. 3: AFF1 regulates ARF19 and ARF7 accumulation.
From: Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
a Confocal images of YFP-ARF19 fluorescence from various tissues of 3d-old wild type (Wt; Col-0) and aff1-1 seedlings carrying 35 S:YFP-ARF19 (false-colored yellow). Three independent experiments were performed with similar results. Scale bar = 50 µm. b Immunoblot analysis of 3d-, 4d-, or 5d-old wild type (Wt; Col-0) or aff1-1 seedlings carrying 35 S:YFP-ARF19. Anti- GFP antibodies were used to detect YFP-ARF19, and anti-HSC70 antibodies were used to detect HSC70 (l.c.; loading control). c Quantification of YFP-ARF19 protein levels of 3d-, 4d-, or 5d-old wild type (Wt; Col-0) or aff1-1 seedlings carrying 35 S:YFP-ARF19. Data are mean ± SD from three independent experiments and gray dots represent the individual values. Different letters indicate individual groups for multiple comparisons with significant differences (one-way ANOVA, Duncan, p  <  0.05). d Immunoblot analysis of HA3-ARF1, ARF7-HA, and YFP-ARF19 in seedlings treated with mock (DMSO) or MG132. Anti-HA antibodies were used to detect HA3-ARF1, ARF7-HA, anti-GFP antibodies were used to detect YFP-ARF19, and anti-HSC70 antibodies were used to detect HSC70 (l.c.; loading control). e Quantification of HA3-ARF1, ARF7-HA, and YFP-ARF19 accumulation in seedlings treated with mock (DMSO) or MG132. Data are mean ± SD from three independent experiments and the gray dots represent the individual values. The statistical significance was determined by a two-sided Student’s t-test (Paired two sample for means). P values = 0.00043 (HA3-ARF1), 0.0093 (ARF7-HA), 0.0019 (YFP-ARF19). **P < 0.01 when compared to the mock. The source data in (b), (c), (d) and (e) are provided as a Source Data file.
