Fig. 4: AFF1 regulates ARF19 and ARF7 protein degradation.
From: Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
a In vitro YFP-ARF19 degradation. Plant lysate from aff1-1 arf19-1 35 S:YFP-ARF19 was incubated with GST, GST-AFF1, or GST-ΔF-box-AFF1 recombinant proteins for the indicated times. Immunoblot analysis (top) and quantification (bottom) of YFP-ARF19, GST, GST-AFF1, or GST-ΔF-box-AFF1 using the indicated antibodies. Anti-HSC70 used for loading control (l.c.). b In vitro ARF7-HA degradation. Plant lysate from aff1-1 35 S:ARF7-HA was incubated with GST, GST-AFF1, or GST-ΔF-box-AFF1 recombinant proteins for the indicated times. Immunoblot analysis (top) and quantification (bottom) of ARF7-HA, GST, GST-AFF1, or GST-ΔF-box-AFF1 using the indicated antibodies. Anti-HSC70 used for loading control (l.c.). Data are mean ± SD of three independent experiments. Different letters indicate individual groups for multiple comparisons with significant differences (one-way ANOVA, Duncan, p  <  0.05) and gray dots represent the individual values. The source data in (a) and (b) are provided as a Source Data file.
