Fig. 4: PNNs are required for extragonadal aromatase regulation of CA1 synaptic inhibition. | Nature Communications

Fig. 4: PNNs are required for extragonadal aromatase regulation of CA1 synaptic inhibition.

From: Sex-specific regulation of inhibition and network activity by local aromatase in the mouse hippocampus

Fig. 4

A Adult intact female mice received daily intraperitoneal (i.p.) injections of aromatase blocker letrozole (LTZ, Arom Block). After 5 days of treatment, perineuronal nets (PNNs) were evaluated quantifying WFA staining intensity. B Representative images showing WFA staining of PNNs (green) surrounding CA1 parvalbumin PV-INs (red) of vehicle (Control) and aromatase blocker letrozole (Arom Block) treated female mice. Scale bar: 20 μm. Graph shows frequency distribution analysis of WFA staining intensities around PV-INs, arbitrary units (arb. units). Aromatase blockade increases WFA staining intensity around female CA1 PV-IN. Two-way ANOVA, Treatment F(1, 12) = 14.88, p = 0.0023; n = 6, 8 mice. C Adult female mice received a single intrahippocampal injection of Chondroitinase ABC (ChABC) or vehicle (Sham) 14 days after ovariectomy (OVX). On the same day of the ChABC or vehicle intracerebral injection, mice received the first intraperitoneal (i.p.) daily injection of aromatase blocker letrozole (Arom Block) or vehicle (C). After 5 days of treatment, CA1 PYR neuron spontaneous Inhibitory Post-Synaptic Currents (sIPSCs) frequency and amplitude were determined. D ChABC treatment prevented the increase of sIPSCs frequency caused by Aromatase blockade (Left graph). Two-way ANOVA, Treatment Arom block F(1, 60) = 8.432, p = 0.0052; treatment ChABC, F(1, 60) = 3.715 × 10−7, p = 0.9995. Bonferroni’s comparison tests, C vs. Arom Block (Sham) p = 0.0045, C vs. Arom Block (ChABC) p > 0,99. No significant changes were observed in sIPSCs amplitude (right graph). Two-way ANOVA, Treatment Arom block F(1, 60) = 0.72, p = 0.4; treatment ChABC, F(1, 60) = 2.78, p = 0.1. Bonferroni’s comparison tests, C-Arom Block (Sham) p = 0.22, C-Arom Block (ChABC) p = 0.97; n = 11, 13, 19, 21 cells from 3 mice per group. Graphs represent mean ± SEM (symbols and bars) and individual values (gray circles). *p < 0.05; ns, p > 0.05. Source data are provided as a Source Data file.

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