Fig. 3: Synthesis of (LNA)-amide-phosphodiester chimeric oligonucleotides. | Nature Communications

Fig. 3: Synthesis of (LNA)-amide-phosphodiester chimeric oligonucleotides.

From: An LNA-amide modification that enhances the cell uptake and activity of phosphorothioate exon-skipping oligonucleotides

Fig. 3

a Efficient solid-phase synthesis of amide-phosphodiester chimaeras. Dashed lines indicate the presence or absence of 2′-4′-methylene bridge. PyBOP benzotriazol-1-yloxytripyrrolidinophosphonium hexafluorophosphate. b Reversed-phase HPLC chromatogram. X-axis = time in minutes. y-axis = absorbance at 260 nm. c Electrospray ES- mass spectrum of a crude LNA-amide chimeric oligonucleotide (ON14). X-axis = mass Y-axis = relative intensity. Required mass = 5438.7 Da, Found mass = 5439.0 Da.

Back to article page