Fig. 5: Volcano plots demonstrate significant enrichment of distinct merozoite proteins in specific experimental conditions.
From: Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria
Each panel shows the abundance of individual proteins after a comparative analysis of the following experimental conditions (x-axis): A ring cultures versus mock-shaved ring cultures, B ring cultures versus uEs in fresh media, C uEs in used media versus uEs in fresh media, and D uEs in used media versus ring cultures. Black and green dots in each panel indicate the Plasmodial and human proteins, respectively that were detected significantly more abundantly in one experimental condition compared to the other. Grey dots indicate proteins that were not detected differentially in the respective experimental conditions. For each panel, subheadings are the suggested localisation of the Plasmodium proteins shown in black. The negative Log10 p-values are displayed on the y-axis and the Log2-fold changes are displayed on the x-axis. The dotted red lines indicate the 0.05% FDR significance cut-off. Differences between pairs of treatment conditions were calculated using two-sample t-tests without correction for multiple comparisons. Four independent biological replicates were analysed for each experimental condition. Source data are uploaded onto the PRIDE database.
