Table 2 Summary of PDS and EIN2 RNAi in RdDM mutant backgrounds.

From: Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants

Constructs

Total no. transgenic lines scored

Silenced lines

Unsilenced/weakly silenced lines

Col/hpPDS[WT]

100

57 (57%)

43

Col/hpPDS[G:U]

172

160 (93%)

12

nrpd1a/hpPDS[WT]

52

46 (88.5%)

6

nrpd1a/hpPDS[G:U]

40

40 (100%)

0

ago4-2/hpPDS[WT]

59

56 (94.9%)

3

ago4-2/hpPDS[G:U]

100

100 (100%)

0

Col/hpEIN2[WT]

20

13 (65%)

7

Col/hpEIN2[G:U]

20

18 (90%)

2

nrpd1a/hpEIN2[WT]

20

20 (100%)

0

nrpd1a/hpEIN2[G:U]

20

20 (100%)

0

ago4-2/hpEIN2[WT]

19

16 (84.2%)

3

ago4-2/hpEIN2[G:U]*

11

10 (90.9%)

1

  1. *A number of lines had poor germination on ACC medium in the darkness, possibly due to extreme EIN2 RNAi, so the plant number is too small for proper comparison with the other transgenic populations.
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