Fig. 2: F. vesca fveagl62 mutant seeds show reduced auxin biosynthesis.
From: Mechanism of fertilization-induced auxin synthesis in the endosperm for seed and fruit development
a Hierarchical clustering of auxin biosynthesis gene expression in WT and fveagl62 stage 2 seeds. b Hierarchical clustering of GA biosynthesis gene expression in WT and fveagl62 stage 2 seeds. c RT-qPCR analysis of five auxin biosynthesis genes in WT and fveagl62 stage 2 seeds. Gene IDs are indicated in a. Y-axis indicates the relative expression level against the control gene FvePP2a (FvH4_4g27700). Significant difference by two-tailed Student’s t-test is indicated by ** (P < 0.01) or * (P < 0.05) between WT and fveagl62 mutant seed. Error bars indicate standard deviation. Similar results were obtained in three biologically independent experiments. d DR5ver2::GUS reporter expression in WT and fveagl62 stage 2 seeds. e DR5ver2::GUS expression in individual stage 2 seeds. f Confocal laser scanning microscopy images of WT and fveagl62 seeds at stage 2. Precocious cellularization of fveagl62 endosperm (arrows) is observed in stage 2 seeds. g Confocal laser scanning microscopic images of WT and fveagl62 stage 2 seeds mock- or NAA-treated. Scale bars: 1 mm (d), 120 μm (e), 100 μm (f–g).
