Fig. 3: Arabidopsis atagl62 endosperms have reduced auxin when compared with WT.
From: Mechanism of fertilization-induced auxin synthesis in the endosperm for seed and fruit development
a Confocal image of auxin reporter R2D2 in WT (Col-0) and atagl62-2 seeds at 2 DPA. Absence of DII-VENUS nuclear signal in the WT endosperm contrasts with strong DII-VENUS nuclear signals in the atagl62-2 endosperm. The scatter graph Y-axis indicates the ratio between DII-VENUS and mDII-Tdtomato signal per endosperm nucleus. A second independent experiment gave a silimar result. The elements of boxplots and source data are provided in the Source Data file. Significant difference indicated by ** (P = 7.55e-12 by two-tailed Student’s t-test) is found between WT and atagl62. b Confocal image of ProAtYUC10::3xnGFP signal in WT and atagl62 endosperms. Note the strong nuclear GFP signals in the WT endosperm and almost undetectable GFP signals in the atagl62 endosperm. c Confocal image of WT and atagl62 mutant seeds at 2 DPA either mock-treated or auxin (2,4-D)-treated. Different extent of endosperm cellularization is observed. Arrows indicate cell walls between endosperm cells. d Quantification of mock- or 2,4-D-treated seeds derived of atagl62-2 (−/+) parents. Y-axis is the percentage of endosperms with one of the three phenotypes: no cellularization, partial cellularization, and complete cellularization. Significant difference (two-tailed Fisher’s exact test) is indicated by ** (P = 0.0006) for complete cellularization or * (P = 0.0416) for no cellularization between mock and 2,4-D treatments of each phenotypic category. e Confocal image of Arabidopsis seeds at 2 DPA. Precocious endoserpm cellularization is absent in transgenic atagl62 plants containing the strawberry FveAGL62 gene. Scale bar in a–e: 50 μm.
