Fig. 5: FveAGL62/FveAGL80 regulates a family of FveATHB genes in strawberry.
From: Mechanism of fertilization-induced auxin synthesis in the endosperm for seed and fruit development
a Hierarchical clustering heatmap showing RNA-seq reads (TPM) in WT and fveagl62 mutant seeds at stage 2 for a subclass of FveATHB genes. Three FveATHB genes (marked by *) are more highly expressed in the fveagl62 mutant seeds than the WT. b Bar graphs showing the expression level (TPM) of three FveATHB genes at the stage 1 ovules, stage 2 seeds, and stage 3 ghosts in WT based on a prior RNA-seq dataset5. c, d Y1H assays showing binding and activation of promoters of FveATHB29b and FveATHB30 by the combined action of FveAGL62 and FveAGL80. e, f Transient repression of the LUC reporter driven by the FveATHB29b (e) or FveATHB30 (f) promoter. Effector proteins are FveAGL62 or FveAGL80 alone or in combination. Significant difference from the negative control (GFP) is marked by ** (P < 0.01 by two-tailed Student’s t-test). Error bars indicate standard deviation. Three biologically independent experiments gave similar results. g Y1H assay testing the binding of FveATHBs to ProFveYUC10::AbAi in the SD-Leu-Ura medium with or without 500 ng/ml AbA. Empty vector containing the AD serves as a negative control. h Transient repression of ProFveYUC10::LUC in tobacco leaves. Y-axis indicates the relative expression level of LUC compared to REN. Significant difference from the negative control (GFP) is marked by * (P < 0.05 by two-tailed Student’s t-test). Error bars indicate standard deviation. Three biologically independent experiments gave similar results.
