Fig. 6: Characterization of FveATHB-OE transgenic lines.
From: Mechanism of fertilization-induced auxin synthesis in the endosperm for seed and fruit development
a Plant and fruit phenotype of FveATHB-OE transgenic lines. Fruits of three FveATHB29b-OE lines and two FveATHB30-OE lines are shown. b Mock- and NAA-treated fruits of FveATHB29b-OE and FveATHB30-OE lines. c Fruit size measurement of WT, three FveATHB29b-OE lines, and two FveATHB30-OE lines. Significant difference from WT is marked by ** (n = 20, P < 0.01 by two-tailed Student’s t-test). The elements of boxplots and source data are provided in a Source Data file. d, e Relative transcript level (Y-axis) of auxin biosynthetic genes FveYUC10 and FveTAA1 in stage 2 seeds of WT and FveATHB29b-OE (d) and FveATHB30-OE (e) lines. Significant difference (two-tailed Student’s t-test) from WT is marked with ** (P < 0.01) and * (P < 0.05). Error bars indicate standard deviation. Three biologically independent experiments gave similar results. f Confocal laser scanning microscopy reveals extensive cell death (red arrowheads) and precocious cellularization (white arrows in enlarged inserts) in the endosperm of stage 2 seeds in FveATHB-OE lines. Scale bars, 1 cm (a, b), 100 μm (f).
