Fig. 3: Pol δ drives recovery from (CG)24 and (CGG)61 stalls, but not (G)50 or (C)50.
From: The mechanism of replication stalling and recovery within repetitive DNA
A Replication reactions carried out with the indicated templates in the absence or presence of pol δ. Pulse-chase experiments carried out with the (CGG)61 template in the absence (B) or presence (C) of pol δ. Reactions were initiated with radiolabelled dATP for 10 min, chased with excess ‘cold’ dATP and samples taken at the indicated time points. D, E Same as in B, C but with the (CG)24 template. F Pulse-chase experiments carried out with the indicated template. Reactions were initiated in the absence of pol δ to generate a pre-existing stall. After a 10 min pulse, pol δ was either added with the chase or not and samples taken at the indicated time points.
