Table 3 Protein purification.

Protein	Strain	Source	Purified as in
Proteins expressed in budding yeast
ORC	ySDORC	Frigola et al.⁸⁶	Frigola et al.⁸⁶
Mcm2-7/Cdt1	yAM33	Coster et al.⁸⁷	Coster et al.⁸⁷
DDK	ySDK8	On et al.⁸⁸	On et al.⁸⁸
CDK	yAE88	Hill et al.⁹¹	Yeeles et al.³⁶
Csm3/Tof1	yAE48	Yeeles et al.³	Yeeles et al.³
Pol epsilon	yAJ2	Yeeles et al.³⁶	Yeeles et al.³⁶
Pol epsilon Exo- (pol2-D290A/E292A)	yAE99	Goswami et al.⁹⁰	Yeeles et al.³⁶
Pol epsilon P301R (pol2-P301R)	ySW1	This study	Yeeles et al.³⁶
Dpb11	yJY26	Yeeles et al.³⁶	Yeeles et al.³⁶
Cdc45	yJY13	Yeeles et al.³⁶	Yeeles et al.³⁶
Mrc1	yAE71	Hill et al.⁹¹	Baretić et al.⁸⁵
RPA	yAE31	Yeeles et al.³⁶	Yeeles et al.³⁶
RFC	yAE41	Yeeles et al.³	Yeeles et al.³
Pol delta	yAE34	Yeeles et al.³	Yeeles et al.³
Pol alpha	yAE95	Hill et al.⁹¹	Yeeles et al.³⁶
Sld3/Sld7	yTD6	Yeeles et al.³⁶	Yeeles et al.³⁶
Dna2	yFV43	Deegan et al.⁸⁴	Deegan et al.⁸⁴
Pif1	pTDK10	Deegan et al.⁸⁴	Deegan et al.84
Pif1 K264A	pTDK24	Deegan et al.⁸⁴	Deegan et al.⁸⁴
TopoI	yAE42	Yeeles et al.³	Yeeles et al.³

Protein	Plasmid	Source	Purified as in
Proteins expressed in bacteria
Cdc6	pAM3	Frigola et al.⁸⁶	Frigola et al.⁸⁶
GINS	pJFDJ5	Yeeles et al.³⁶	Yeeles et al.³⁶
Mcm10	pMD132	Douglas & Diffley⁸⁹	Douglas & Diffley⁸⁹
PCNA	pJY19	Yeeles et al.³	Yeeles et al.³
Sld2	pGC441	This study	Detailed in “Methods” section

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