Fig. 3: Brightness comparison of BRIC and Orange CaMBI 110 (OCaMBI110) in the hippocampus in live mice.

a Left: Illustration of stereotactic intracranial administration of AAVs. Right: Image of an acute brain slice prepared from a BRIC-transduced mouse, showing the successful expression of the indicator in the hippocampus. The fluorescence channel (red) is overlaid on the corresponding grayscale brightfield image. Scale bar, 2 mm. b Representative bioluminescence images of live mice with the hippocampus transduced with BRIC or OCaMBI110 AAVs. The substrates were administered via tail vein at their respective saturation concentrations. Images with peak bioluminescence intensities were presented in pseudocolor overlaid on corresponding brightfield images. c Bioluminescence intensity over time presented for each substrate. The inset shows the same results with a logarithmic y-axis. d Comparison of the integrated bioluminescence intensity (area under the curve) with the residual background subtracted. Data are presented as mean ± s.e.m. (n = 4 mice). P values were derived from ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. The GraphPad Prism software does not provide extract P values below 0.0001. BL, bioluminescence. Arb. units, arbitrary units. Source data are provided as a Source Data file.