Fig. 4: BLI of Ca²⁺ dynamics in the brain in awake mice.

a Illustration of intracranial viral administration and BLI of head-fixed awake mice. b Footshock-induced Ca²⁺ in the basolateral amygdala (BLA). Top: Viral injection sites and the general experimental procedure. Middle: Bioluminescence intensity heatmap of a representative BRIC- or BREP-expressing mouse in response to 13 consecutive trials of footshock stimulations. Bottom: Quantification of intensity changes presented as mean ± s.e.m. (n = 4 mice, each with 13 trials). The right panel compares the average responses of 13 trials for each mouse. The P value was derived from paired two-tailed t-tests. c Ca²⁺ in the hippocampus (HPC) during kainic acid (KA)-induced seizures. Top: Viral injection sites and the general experimental procedure. Middle: Intensity traces of BRIC- or BREP-expressing mice. Bottom: Comparison of maximal intensity changes and area under the curve (AUC) presented as mean ± s.e.m. (n = 6 mice). P values were derived from two-way ANOVA followed by Šídák’s multiple comparisons test or unpaired two-tailed t-tests. The baselines for all intensity traces were corrected using a monoexponential decay model. BL, bioluminescence. Arb. units, arbitrary units. Source data are provided as a Source Data file.