Table 1 Properties of bioluminescence Ca²⁺ indicators based on NanoLuc or NanoLuc-derived luciferases^a.

Construct	Peak emission (nm)^b	BL/BL₀^c	K_d (nM)	Emission fraction > 600 nm	BRET Donor	BRET Acceptor	Ca²⁺ sensing domain	Reference
GeNLs(Ca²⁺)	517	3.9–5	60–520	0.017^d	NLuc split at residue 66	mNeonGreen	CaM, M13	²⁵
CalFluxVTN	525	5^e	480	0.04^d	NLuc	Venus	TnC	¹³
LUCI-GECO1	515	2.6^e	285	<0.02	NLuc	ncpGCaMP6s	CaM, RS20	²⁶
GLICO	515	23	230	<0.02	NanoBiT	GCaMP6f	CaM, RS20	²⁷
ReLICO	452	3.4	1.5 × 10⁶	<0.02	NanoBiT	R-CEPIA1er	CaM, RS20	²⁷
Orange CaMBIs	586	7–8 ^f	110–300	0.33^d	NLuc split at residue 133	CyOFP1	CaM, M13	²⁹
BRIC	595	6.5	133	0.54	teLuc split at residue 133	mScarlet-I	CaM, M13	This work

^aData for BRIC were determined in this work. Unless otherwise indicated, other data were reported or calculated from graphs in the initial publications. ^bWavelength for the major or the most red-shifted emission peak. ^cIntensity ratio with or without Ca²⁺ at the indicated peak emission wavelength. ^dAdapted from Reference ²⁹. ^eOriginally described as green/blue ratiometric indicators, and the response range (R/R₀) was reported to be 11 and 5 for CalFluxVTN and LUCI-GECO, respectively. ^fOur determined value is ∼2.5–3.7 for OCaMBI110. The discrepancy may be caused by expression and assay conditions and the variable oligomerization states of Orange CAMBIs.

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