Fig. 4: MEK mutations alter the spatiotemporal dynamics of ERK signaling. | Nature Communications

Fig. 4: MEK mutations alter the spatiotemporal dynamics of ERK signaling.

From: Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer

Fig. 4

a, b Disease-associated MEK mutants differentially affect ERK signaling. a HEK293 cells stably expressing HA-MEK1 WT ( + ), F53S, or K57N were treated with EGF for the indicated times. The phosphorylation levels of Raf1, MEK1, ERK1/2, and rpS6 were analyzed by immunoblotting using appropriate phospho-specific Abs. The expression levels of Raf1, MEK1, ERK1/2, rpS6, and Egr1 in cell lysates are also shown. b The intensity of the P-ERK1/2 (top), P-rpS6 (middle), and Egr1 (bottom) bands in a was quantified. c, d The indicated cells stably expressing ERK1-GFP were treated with EGF for the indicated times. c ERK1 localization was visualized by GFP fluorescence. Scale bar, 10 μm. d Time course of the changes in the nuclear localization of ERK following EGF treatment. The percentage of nuclear fluorescence relative to that in the whole cell was calculated. Data are mean ± SEM from five independent experiments. P-values were determined using one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05; **p < 0.01. Source data are provided as a Source Data file.

Back to article page