Fig. 1: Identification and phenotypes of Arabidopsis dph1 mutants.
From: Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis
a Scheme of first step of diphthamide biosynthesis in yeast and human. SAM S-adenosylmethionine, MTA 5′-methylthioadenosine. b Arabidopsis hygromycin sensitivity. Photograph of 12-day-old seedlings of the wild-type (WT), dph1-1, dph1-2, and dph1-1 pDPH1:DPH1-GFP complemented line Y22-10 cultivated without (control) or with 2 μM hygromycin B (hyg). c–e Growth of WT, dph1, and complementation lines on 0.5× MS medium, shown as photograph (c), fresh biomass (d), and primary root length (e) of 12-day-old seedlings. Scale bar, 10 mm (c). Data are mean ± s.d., n = 4 pools of between 5 and 6 seedlings, with each pool sampled from an independent petri plate (d, e). Significant differences from WT: *P < 0.05, ***P < 0.001 (one-way ANOVA, Tukey’s test).
