Fig. 3: The change in Stat92E pairing states does not reflect the difference in cell-cycle stages.

a A representative image of Stat92E nascent transcript (red) in Fly-Fucci testis. GFP-E2F1-230 (blue) is positive in G2-phase and negative in S-phase cells. White dotted lines encircle germ cells in indicated cell cycle stages. DAPI is shown in green. Right panel shows GFP channel. Stat92E nascent transcript was typically weak or undetectable in S-phase cells. b Representative images of G2 phase germ cells in indicated stages. Stat92E intron FISH signal (white arrowheads) indicate pairing states at the indicated stage of germ cells. Representative pairing states are shown in the upper right corner of each image. Lower panels show GFP channel. c Violin plots show distances between Stat92E nascent transcript puncta at the indicated stages of germ cell development measured in all cell-cycle stages or only in G2-phase cells. Violin plots show KDE and quantile lines and the width of each curve corresponds with the frequency of data points. The adjusted p values were calculated with Šidák’s multiple comparisons. All plotted data points are provided in Source Data. Number of scored cells, which are randomly chosen from at least 10 testes for each experiments, is shown for each data point. Scale bars in (a) represent 10 μm. Scale bars in b represent 2 μm.