Fig. 4: The change in Stat92E pairing states is required for subsequent silencing of transcription. | Nature Communications

Fig. 4: The change in Stat92E pairing states is required for subsequent silencing of transcription.

From: Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation

Fig. 4

a A schematic of BacTg/Df(3 R)BSC516 (BacTg/Df) genotype. b Representative images of Stat92E DNA FISH puncta (red, white arrowheads) at the indicated stages of germ cells (Vasa, cyan) in BacTg/Df genotype. c Violin plots show distances between DNA FISH puncta. d Representative images of Stat92E mRNA (exon probe smFISH, green) at the indicated stages of germ cells in indicated genotypes. e Quantification of Stat92E mRNA levels. Y axis values are the number of mRNA dots present in a middle plane of the cell (dot#/plane). SIs (see text) are shown in boxes above bars. f Representative images of Stat92E nascent transcript (intron probe, red) at the indicated stages of germ cells in BacTg/Df testes. DAPI (cyan). g, h, i Representative images of Stat92E DNA FISH (red, white arrowheads) at the indicated stages of germ cells (Vasa, cyan) in indicated genotypes. j Violin plots show distances between DNA FISH puncta. k Quantification of Stat92E mRNA levels. Y axis values are the number of mRNA dots scored in a middle plane of the cell (dot#/plane). SIs (see text) are shown in boxes above bars. Adjusted p values are provided for comparison between control (wild type, yw) and BacTg/Df data in (c) and (e) and comparison between temperature-shifted experiment (temp shift) and no temperature-shift control (no temp shift) in (j) and (k). The adjusted p values were calculated using Šidák’s multiple comparisons. Violin plots show KDE and quantile lines and the width of each curve corresponds with the frequency of data points. Box plots show 25–75% (box), median (band inside) and minimum to maximum (whiskers) with all data points. All plotted data points are provided in Source Data. Number of scored cells, which are randomly chosen from at least 10 testes for each experiments, is shown for each data point. For RNAi experiments, temperature sensitive nosGal4 driver, nosGal4ts, was used. All scale bars represent 2 μm. Representative pairing states are shown in lower left corner of each image.

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