Fig. 5: Stat92E nascent transcript level differs in paired and unpaired conditions.

a Schematic of cell cycle stages of a GSC and GB pair. b Representative images of Stat92E nascent transcripts in GBs. Left and right panel show paired or unpaired Stat92E homologous alleles, respectively. Red arrowheads indicate Stat92E nascent transcripts. c Quantification of nascent transcript intensity (measured as a ratio relative to nascent transcript intensity of CySC’s, see Method for details) in paired and unpaired conditions of GBs. Left two columns show intensity/punctum. Right two columns show intensity/allele. To plot intensity/allele, measured intensities of paired cases were divided by two. d Representative images of Stat92E DNA FISH in GBs. Left and right panel show paired or unpaired Stat92E homologous alleles, respectively. Red arrowheads indicate Stat92E locus visualized by DNA FISH. e Quantification of DNA FISH signal intensity in paired and unpaired conditions of GBs. Left two columns show intensity/punctum. Right two columns show intensity/allele. To plot intensity/allele, measured intensities of paired cases were divided by two. f A schematic of the intensity measurement results shown in (c) and (e). Box plots show 25–75% (box), median (band inside) and minimum to maximum (whiskers) with all data points. The p values were calculated by two-tailed Student’s t-tests. All scale bars represent 2 μm. Number of scored cells, which are randomly chosen from at least 10 testes for each experiments, is shown for each data point.