Fig. 1: Schematics of CRISPR-Cas9 activated graphene biointerfaces for extraction and real-time in vivo monitoring of universal cfDNA. | Nature Communications

Fig. 1: Schematics of CRISPR-Cas9 activated graphene biointerfaces for extraction and real-time in vivo monitoring of universal cfDNA.

From: Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA

Fig. 1

a Workflow of the wearable patch fabrication. b Scheme showing CRISPR microneedles integrated with the dCas9 enzyme and a sequence-specific sgRNA (denoted as dRNP) immobilized on a carboxyl graphene surface. c Real-time monitoring of the enriched cfDNA based on reverse iontophoresis and CRISPR-Cas9 activated graphene biointerfaces. The specific recognition of cfDNA to dRNP regulates the electrochemical characteristics and potential difference of the graphene layer, generating electrical signal output.

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