Fig. 1: Comparison of parasitemia of non-adapted and adapted P. falciparum strains in RBC-NSG mice. | Nature Communications

Fig. 1: Comparison of parasitemia of non-adapted and adapted P. falciparum strains in RBC-NSG mice.

From: Selective expression of variant surface antigens enables Plasmodium falciparum to evade immune clearance in vivo

Fig. 1

a RBC-NSG mice were infected with the indicated strains of non-adapted or adapted (AS) P. falciparum strains, and parasitemia was assessed by Giemsa staining of peripheral blood smears. Black line shows parasitemia levels (tracked till Day 15) of RBC-NSG mice directly infected with whole blood, including iRBCs, from RBC-NSG mice that had been infected with non-adpated 3D7 parasite 25 day earlier and was parasitemic. Each point represents the parasitemia of each mouse’s respective parasite strain. b A single clone of 3D7, 3D7-B7, was isolated, expanded, and used to infect RBC-NSG mice. After 40 days, parasites from peripheral blood were recovered, cultured, and expanded in vitro to generate 3D7-B7 AS. 3D7-B7 AS parasites were then used to infect a new batch of RBC-NSG mice. Each point represents the mean ± SD of n = 3 biologically independent experiments. Source data are provided as a Source Data file.

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