Fig. 5: LILRB1 inhibits NK cell killing of iRBC but not macrophage phagocytosis. | Nature Communications

Fig. 5: LILRB1 inhibits NK cell killing of iRBC but not macrophage phagocytosis.

From: Selective expression of variant surface antigens enables Plasmodium falciparum to evade immune clearance in vivo

Fig. 5

a NK cells were co-cultured with either non-adapted 3D7attB or adapted 3D7attB AS parasites for 96 hrs and parasitemia was quantified by flow cytometry. Parasitemia reduction was calculated as described in materials and methods. n = 3 biologically independent samples, paired T test, *p =  0.0333. b NK cells were incubated with either an anti-LILRB1 neutralizing antibody or an isotype control and then co-cultured with either 3D7attB or 3D7attB AS parasites for 96 hrs. Parasitemia was quantified by flow cytometry. Data shown are mean ± SD, n = 3 biologically independent samples. One-way ANOVA with Tukey’s multiple comparisons, *p = 0.0342, **p = 0.0023. c Macrophage phagocytosis assay of 3D7, 3D7 AS, PfEMP1 PF3D7_0421300-expressing 3D7 (3D7-SLI-PfEMP1) parasites and RIFIN PF3D7_1254800-expressing 3D7 (3D7-SLI-RIFIN). Data shown are mean ± SEM, n = 3 biologically independent samples, One-way ANOVA with Tukey’s multiple comparisons, *p = 0.0109, **p = 0.0036, ***p = 0.0008. Source data are provided as a Source Data file.

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