Table 1 List of parasites adapted and used.
Parasite | Source | Description | Day post infection for adapted parasite to appear in peripheral blood |
|---|---|---|---|
3D7 | BEI Resources (MRA-102) | Clone derived from NF54 by limiting dilution61. | 3D7-B7 AS – Day 27 |
W2mef | BEI Resources (MRA-615) | Mefloquine-resistant culture lines derived from Indochina strain62. | W2mef AS – Day 19 |
T994 | BEI Resources (MRA-153) | Isolate derived from T9 strain collected from patient in Thailand63. | T994 AS – Day 21 |
NF54attB | Niles lab | NF54attB that harbors an attB site at the Cg6 locus con Chr 764. | NF54attB AS – Day 24 |
3D7attB | BEI Resources (MRA-845) | 3D7 parasite that harbors an attB site at the Cg6 locus on Chr 723. | 3D7attB AS – Day 30 |
NF54attBCas9+T7 Polymerase (NF54CR) | Niles lab | NF54 parasites that expressed Cas9 and T7 RNA polymerase65. | NF54CR AS – Day 25 |
NF54CR AS mahrp1 | This paper | mahrp1 gene under the regulation of aTc on the NF54CR AS background. | Adapted parasites used for transfection |
3D7-SLI-RIFIN* | This paper | 3D7 strain with PF3D7_1254800 gene tagged by a resistant marker for continuous expression. | N.A. |
3D7-SLI-PfEMP1* | This paper | 3D7 strain with PF3D7_0421300 gene tagged by a resistant marker for continuous expression. | N.A. |
