Fig. 1: HNRNPU is strongly expressed in mitotic mouse neural progenitors and is essential for brain development.
From: Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the developing mouse cortex
A-C’ Expression pattern of HNRNPU (Red) in coronal sections of E13 embryos cortices. A EdU, (Green), 1 h post-injection highlights cells in the S-phase at the basal border of the ventricular zone. B Acetylated tubulin (green) marks stabilized tubulin at the radial progenitor primary cilia. C CENP-B (green) marks centrosomes tethered to the apical surface. D, E Flow Cytometry of dissociated neurospheres (E13, 2 days in vitro) at four stages of the cell cycle, classified by EdU incorporation and DAPI stain. Red- HNRNPU, blue-DAPI. E Distribution of the levels of HNRNPU and co-localization with DAPI (Similarity) in a population of dissociated neurospheres classified as G2/M n = 165 or G1/S n = 1525. Images of whole brains and corresponding 5 μM thick coronal sections (Nissl staining) of embryonic day 18 (E18, F, G) and postnatal day 8 (P8), of Emx1Cre littermates carrying WT (F, H) or floxed Hnrnpu alleles (G, I). J–O Expression of GFAP (Red) and Tbr1 (Green) in section of E18 obtained from control (Hnrnpu +/+ Emx1Cre/+) and mutant (Hnrnpu fl/fl Emx1 Cre/+) littermates. Insert indicate region magnified in L, O. Tbr1 images (K, N) were captured from equivalent locations. P Averaged GFAP intensity histograms in arbitrary units after background subtraction along the upper 300 μM of coronal sections of E18 cortices (n = 3, each measured in triplicates). Q–V P21 brain sections of mutant and control (Hnrnpu +/+ Emx1 Cre/+) littermates stained with cortical plate layers markers (Tbr1, Cux1 in Q, T) and glia markers, 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase R, U) and GFAP (S, V). Schematic representation of the slice morphology (pink) showing images location (black rectangle). LV-lateral ventricle, CPu- caudate putamen, CTX- Cortex. Size bars are in μM.
