Fig. 2: Neural progenitors are highly sensitive to HNRNPU loss.
From: Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the developing mouse cortex
Dual Color Cre reporter (CAG::SL) was electroporated to E13 Hnrnpu fl/fl embryos, injected with either no Cre (A–C, N-0, Supp_Movie 1) or with Cre expressing plasmids under one of three promoters (CAG::NLS-Cre-GFP (D–F, P, Q, Supp_Movie 2), Tbr2::Cre and (G–I, R, S, Supp_Movie 3) Ta1::Cre (J–L, T–U, Supp_Movie 4) A–L Images of time-lapse recording (5 h long) of primary cultures prepared from electroporated cortices. Arrowhead highlight specific cells in the field of view. M Tracking of cell viability in each field of view, a dot indicates the last time point a tracked cell is visible before disintegration, vertical lines indicate average values, bars indicate ±SEM range. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparison tests, p < 0.0001. Control, n = 17, CAG::NLS-Cre-GFP n = 14, Tbr2::Cre, n = 9 and Ta1::Cre n = 10. N–U Images of fixed primary cultures, after 2 days in vitro (2DIV), Showing transfected cells (expressing floxed ZsGreen, Green) and cells with an excised reporter (mCherry, Red). V Percentage of cells expressing mCherry out of ZsGreen expressing cells in Control (N, O, n = 581) CAG::NLS-Cre (P, Q, n = 787), Tbr2::Cre (R, S n = 182) and Ta1::Cre (T, U, n = 137). Blue, DAPI. Statistical analysis was done using ordinary one-way ANOVA with uncorrected Fisher’s LSD, p values: no Cre vs. CAG::NLS-Cre 0.0092, No cre vs. Tbr2::Cre p = 0.0056. No Cre vs. Ta1::Cre p < 0.0001. CAG::NLS-Cre vs. Tbr2::Cre, NS. CAG::NLS-Cre vs. Ta1::Cre p < 0.0001. Tbr2::Cre vs. Ta1::Cre p < 0.0001. **P < 0.01, ***P < 0.001, ****P < 0.0001, ns-non significant. Variability is indicated by ±SEM bars. Size bars units are μM. Source data are provided as a Source Data file.
