Fig. 6: DEIP1 co-migrates with core subunits of the Cytb6f complex.
2D-SDS-PAGE analyses of thylakoids purified from a wild-type plants grown under moderate (ML) light for 7 days, b deip1-1 mutants grown under low light (LL) for 5 weeks, and c the deip1-C4 complemented line grown under moderate light (ML) for 7 days. Thylakoid samples equivalent to 20 µg chlorophyll were solubilized in β-DDM, and resolved in an 8–13.8% gradient gel. SDS-PAGE was performed as second dimension, and immunodetection was conducted with anti-PetA, anti-PetB, anti-PETC, and anti-PetD antibodies. For the deip1-C4 line, the antibody against GFP was included, and the band that corresponds to DEIP1-GFP is indicated by the black arrowhead. Putative assembly intermediates are indicated as I, II, III, IV, V, and VI. See text for details. n = 3 independent biological replicates.
