Fig. 7: DEIP1 is associated in high molecular weight complexes and interacts primarily with PetA and PetB. | Nature Communications

Fig. 7: DEIP1 is associated in high molecular weight complexes and interacts primarily with PetA and PetB.

From: De-etiolation-induced protein 1 (DEIP1) mediates assembly of the cytochrome b6f complex in Arabidopsis

Fig. 7

a BS3-mediated crosslink of stroma fractions and thylakoid samples obtained from transgenic plants expressing a soluble chloroplast-targeted GFP (ctpGFP) and the deip1-C4 line. The treated samples were separated in a 10% SDS PAA gel. An anti-GFP antibody was used to detect the soluble GFP, the monomeric DEIP1 (mDEIP1-GFP) and the high molecular weight complexes containing DEIP1 (hmwDEIP1-GFP). n = 3 independent biological replicates. b Co-IP from deip1-C4 and the wild type (WT). Thylakoid samples were treated with 1.5 mM DSP crosslinker prior to co-IP. The eluate was resolved by SDS-PAGE, and the immunodetection was done with anti-GFP, anti-PetA, anti-PetB, anti-PETC, anti-PetD, anti-PsbA, anti-AtpB and anti-PsaC antibodies. n = 3 independent biological replicates.

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