Fig. 8: BiFC analyses to verify the interaction of DEIP1 with Cytb6f complex subunits in chloroplasts. | Nature Communications

Fig. 8: BiFC analyses to verify the interaction of DEIP1 with Cytb6f complex subunits in chloroplasts.

From: De-etiolation-induced protein 1 (DEIP1) mediates assembly of the cytochrome b6f complex in Arabidopsis

Fig. 8

Bimolecular fluorescence complementation (BiFC) assays with the DEIP1-cYFP fusion protein in tobacco protoplasts are shown. Interactions were assessed with the nYFP fused to the C-terminus of DEIP1, PetA, PetB, PETC and PetD. The endogenous transit peptide was employed for DEIP1, and the pea RBCS chloroplast transit peptide was employed for all Cytb6f subunits. The bright field (BF), YFP (green), chlorophyll auto fluorescence (red) and the merged images are depicted. Representative images were selected based on the observation of 80 protoplasts in two independent experiments for each interaction. Scale bars: 20 μm.

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