Fig. 1: Isolation, characterization, and analysis of lung metastatic derivatives.
a Construction of the multi-organ metastasis model and screening of organospecific metastatic cell derivatives. I.C., intracardiac. b Hematein-eosin (H&E) staining of lung, bone, and brain metastases (arrowheads) by Rencaluci cells. Scale bar, 400 μm. c Flowchart of the in vivo selection of lung, bone, and brain-specific metastatic subpopulations from Rencaluci cells. d Kaplan–Meier survival curves for lung metastasis-free survival of Rencaluci cells and representative derivatives (Parental, n = 10 mice; LuM2b, n = 8 mice; BoM2, n = 9 mice; BrM2b, n = 8 mice). e Kaplan–Meier survival curves for overall survival of RencaParental and RencaLuM2b cells (Parental, n = 10 mice; LuM2b, n = 8 mice). f Representative ex vivo bioluminescent and H&E images of lung metastases at 30 days after I.C. injection of Rencaluci cells (Parental, n = 9 mice; LuM2b, n = 8 mice; left panel) and quantification of the photon flux (right panel). Scale bar, 400 μm. g Representative ex vivo bioluminescent images of lung metastases at 90 days after I.C. injection of 786Oluci/eGFP cells (Parental, n = 5 mice; LuM1a, n = 6 mice; left panel) and quantification of the photon flux (right panel). In f and g, the data are presented as mean ± SEM. Two-sided log-rank test was used for statistical analysis of (d) and (e), two-tailed Student’s unpaired t-test for (f) and (g). Source data are provided as a Source data file.
