Fig. 3: LAPTM5 promotes self-renewal and cancer stem cell traits of RCC cells.
a Schematic illustration for the steps of cell inoculation and lung metastases detection. I.V., intravenous. b Representative IHC images of luciferase staining in lung sections harvested from mice treated as in (a). Scale bar, 50 μm. Quantification of micrometastasis (diameter < 100 μm, c) and macrometastasis (diameter ≥ 100 μm, d) per lung section in (b) (n = 5 mice per group). Tumor sphere assay (e) and quantification of tumor sphere formation of indicated cells (f, n = 3 per group). Scale bar, 200 μm. g Frequencies of tumors formation in indicated groups. Tumor volumes of control and Laptm5-overexpressing Renca cells (h, n = 11 mice per group) or control and Laptm5-silenced RencaLuM2b cells (i, n = 10 mice per group) inoculated subcutaneously with indicated cell number. j, k qRT-PCR analysis of stemness markers in control and LAPTM5-overexpressing Renca (j) and 786-O cells (k). n = 3 per group. In c, d, the data are presented as whisker plots: midline, median; box, 25–75th percentile; whisker, minimum to maximum values, in (e), (j), and (k), the data are presents as mean ± SD, in (h) and (i), the data are presents as mean ± SEM. Two-tailed Student’s unpaired t-test was used for statistical analysis in (c)–(e), (h)–(k), χ2 test for (g). Source data are provided as a Source data file.
