Fig. 6: WWP2 promotes lysosome-based polyubiquitylation and degradation of BMPR1A. | Nature Communications

Fig. 6: WWP2 promotes lysosome-based polyubiquitylation and degradation of BMPR1A.

From: Lysosomal protein transmembrane 5 promotes lung-specific metastasis by regulating BMPR1A lysosomal degradation

Fig. 6

a IP and IB analyses of 786-O cells transfected with expression vectors for His-WWP2, Myc-BMPR1A and HA-ubiquitin (Ub). b IP and IB analyses of 786-O cells transfected with expression vectors for His-tagged wild type (wt) or C838A mutated WWP2, Myc-BMPR1A and HA-Ub. c IP and IB analyses of 786-O cells transfected with siRNAs against WWP2 or expression vectors for Myc-BMPR1A and HA-Ub. d IP and IB analyses of 786-O cells transfected with expression vectors for Flag-LAPTM5, His-WWP2, Myc-BMPR1A, HA-Ub and treated with different concentrations of chloroquine (CQ) for 60 min. e Representative H&E images of lung metastases at 21 days after I.V. injection of Renca cells stably expressing Laptm5 and/or Bmpr1a (left panel) and quantification of the lung metastatic lesions per slide (right panel, n = 8 mice per group). Data are presented as whisker plots: midline, median; box, 25–75th percentile; whisker, minimum to maximum values. Two-tailed Student’s unpaired t-test was used for statistical analysis. Immunoblots are representative of three biological replicates. Source data are provided as a Source data file.

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