Fig. 5: Lower magnification of striatal-PFF injected mice brain section with AAV-EGFP and AAV-EGFP-PFFNB2 transduction.
From: α-Synuclein fibril-specific nanobody reduces prion-like α-synuclein spreading in mice
Both EGFP and EGFP-PFFNB2 expression (green) were mainly expressed in the motor cortex and somatosensory cortical regions. a Schematics representing the PFF injection site (yellow box) and site of analyses (red box) (b) The ×4 magnification images of the motor cortical region (top panel) and marked regions R1, R2, and R3 (white boxes) are shown in the bottom panel. Scale bars, 100 μm. c The ×4 magnification images of the somatosensory cortical region (top panel) and marked area (white box) is shown in the bottom panel. All experiments were replicated in three mice per group with similar results. Scale bars, 100 μm. d Correlation between EGFP intensity to pS129 intensity plot in the AAV-EGFP and AAV-EGFP-PFFNB2 groups. Areas with low to high degrees of EGFP expression were selected for quantification. There is a negative correlation between EGFP intensity and pS129 intensity in the AAV-EGFP-PFFNB2 group, with the Pearson correlation coefficient r = −0.8490. There is no correlation between EGFP and pS129 in the AAV-EGFP group with r = 0.3414. Source data are provided as a Source Data file.
