Fig. 7: SOX17 regulates Pdgfb expression by directly binding to Pdgfb distal enhancer.
From: A SOX17-PDGFB signaling axis regulates aortic root development
a UCSC Genome browser tracks show two Pdgfb transcriptional enhancers containing the consensus SOX17 binding motifs, one located at the ~10 Kb upstream of the first exon of Pdgfb (enhancer 1) and the other between exon 2 and 3 (enhancer 2) identified for E14.5 and adult mouse hearts based on the mouse ENCODE project. b ChIP-qPCR shows the binding of SOX17 at enhancer 1. (n = 3/group, mean ± SD, unpaired two-tailed t-test, p = 0.03 for enhancer 1, *p < 0.05). c Reporter gene assays show that enhancer 1 activates the promoter of reporter gene and the mutagenesis analysis indicates that the activation is dependent on the intact SOX17 binding motifs. (n = 4/group, mean ± SD, unpaired two-tailed t-test, p = 0.000003 for enhancer 1-WT, p = 0.002 for enhancer 1-Del, *p < 0.05). Source data are provided as a Source Data file.
