Fig. 8: PDGFB re-expression rescues aortic root defects.
From: A SOX17-PDGFB signaling axis regulates aortic root development
a Representative RNAscope images (n = 3/group) of E13.5 control and Sox17eKO;R26RePDGFB hearts showing the human PDGFB expression (red) in aortic endothelium in the Sox17eKO;R26RePDGFB heart. b, c H&E staining (n=5/group) showing normal ventricular wall and NCL, respectively, in all 5 E16.5 Sox17eKO;R26RePDGFB embryos examined. LV/RV: left and right ventricle. d Quantitative analysis shows comparable NCL area in E16.5 Sox17eKO;R26RePDGFB and control hearts. n = 5/group, mean ± SD, unpaired two-tailed t-test, ns, no significance. e Representative H&E stained continuous sections E16.5 control and Sox17eKO;R26RePDGFB hearts from the base of aortic valve leaflets to the coronary ostia showing that LCO is correctly positioned in E16.5 Sox17eKO;R26RePDGFB hearts compared to the controls. Arrowheads indicate the main coronary arteries and ostia. f Quantitative analysis showing no difference in the LCO position related to aortic valve base between Sox17eKO;R26RePDGFB hearts and controls. n = 5/group, mean ± SD, unpaired two-tailed t-test, ns, no significance. g, h H&E staining and quantitative analysis show the normal aortic wall thickness in E16.5 Sox17eKO;R26RePDGFB hearts. n = 5/group, mean ± SD, unpaired two-tailed t-test, ns, no significance. i Representative hypoxia probe staining images (n = 5/group) showing the similar staining pattern and intensity between E16.5 control and Sox17eKO;R26P hearts. Source data are provided as a Source Data file. Scale bars: 100 µm in a, c, e, g; 200 µm in b, i.
