Fig. 9: PDGFB re-expression rescues reduced cell proliferation of VSMCs and NCL and decreased pERK activity resulted from the Sox17 deletion.
From: A SOX17-PDGFB signaling axis regulates aortic root development
a, b EdU (green) assay and quantitative analysis showing the reduced proliferation of VSMCs in the aortic root wall marked by ELSTAIN (red) staining in the Sox17eKO hearts was rescued in the Sox17eKO;R26RePDGFB heart. IB4 (white) stains the endothelium. n = 5/group, mean ± SD, one-way ANOVA Tukey’s test, p = 0.00002 for Sox17eKO, p = 0.00006 for Sox17eKO;R26RePDGFB, *p < 0.05). c, d EdU (green) assay and quantitative analysis showing the reduced proliferation of NCL in the Sox17eKO hearts was rescued in the Sox17eKO;R26RePDGFB hearts. n = 5/group, mean ± SD, one-way ANOVA Tukey’s test, p = 0.001 for NCL of Sox17eKO, p = 0.008 for NCL of Sox17eKO;R26RePDGFB, *p < 0.05. e, f IF images and quantitative analysis showing the reduced phosphorylated ERK (pERK) density (red) in the NCL mesenchyme of the Sox17eKO hearts was restored in the Sox17eKO;R26RePDGFB heart. n = 5/group, mean ± SD, one-way ANOVA Tukey’s test, p = 0.000004 for NCL of Sox17eKO, p = 0.0006 for NCL of Sox17eKO;R26RePDGFB, *p < 0.05. Source data are provided as a Source Data file. Scale bars: 100 µm.
