Fig. 8: ALC1’s ATPase activity stimulates XPC-dependent DNA repair.
From: XPC–PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair
a Quantification of 6-4PP levels based on slotblot in U2OS (FRT) WT, XPC-KO, and ALC1-KO cells at different time points after UV-C damage (20 J/m2). The data is depicted as mean + S.E.M. of four independent experiments. Representative dot blots are shown in Fig. S5a. b Quantification of CPD levels based on slotblot in U2OS (FRT) WT, XPC-KO, and ALC1-KO cells at different time points after UV-C damage (20 J/m2). The data is depicted as mean + S.E.M. of four independent experiments where each experiment is based on two technical replicates. Representative dot blots are shown in Fig. S5a. c, d Clonogenic survival assays of c U2OS (FRT) WT, ALC1-KO, DDB2-KO, and XPC-KO cells as well as d U2OS (FRT) WT, ALC1-KO, ALC1-KO + GFP-ALC1, ALC1-KO + GFP-ALC1 E175Q, ALC1-KO + GFP-ALC1 Δmacrodomain cells upon UV-C irradiation. The data are depicted as mean + S.E.M. from three independent experiments. e Representative images and f quantification of unscheduled DNA synthesis experiments in U2OS (FRT) WT, XPC-KO, ALC1-KO, ALC1-KO + GFP-ALC1, ALC1-KO + GFP-ALC1 E175Q, ALC1-KO + GFP-ALC1 Δmacrodomain cells upon UV-C irradiation. >39 cells were analyzed in four independent experiments. All cells are depicted as individual data points (gray). The median of each biological replicate is depicted as a colored point, whereas the bar represents the median of all data points. The scale bar in e is 5 µm.
