Fig. 3: Characterization of CF iPSC-derived airway epithelial spheroids in terms of morphology, size, and FIS with and without CFTR modulator treatment.
From: A multimodal iPSC platform for cystic fibrosis drug testing
A Representative microscopy images (of n ≥ 3 biological replicates from independent differentiations for each cell line) of airway spheroids from CF and non-CF donors demonstrate differences in morphologic appearance; dashed boxes depict magnified views. B Baseline spheroid size differs with CFTR genotype. Each point represents average spheroid size before forskolin stimulation within an individual experiment (biological replicates from independent differentiations: n = 12 for non-CF, n = 6 for G551D, n = 26 for Phe508del, n = 12 for PTCs). C Representative spheroid images (of n > 3 biological replicates from independent differentiations for each cell line) for the CFTR variants indicated before (0 h) and after (24 h) treatment with compounds shown at bottom of panel. D Change in CSA after treatment of CF airway epithelial cell spheroids with CFTR modulators (n > 3 biological replicates from independent differentiations with exception of n = 2 for G551D #2 VX-770 treatment). P values were calculated using paired two-tailed Student’s t test comparing the treatment sample to forskolin control. Scale bars represent 250 µm. Lines and error bars represent mean ± standard error.
