Fig. 1: The requirement of SutA domains in RNAP interaction and transcription activation.
From: Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding
a SutA domain scheme annotated with predicted secondary structure features. NTD, N terminal domain; WL, wedge loop; RBD, RNAP-binding domain; CTD, C-terminal domain. b Interaction between the SutA domains and RNAP-β protrusion analyzed by the YTH assay. AD, activation domain of GAL4; BD, DNA-binding domain of GAL4. c SutA activates transcription of the RNAP-σS holoenzyme (left) but has much less effect on RNAP-σA holoenzyme (right) in a fluorescence-based in vitro transcription assay. Truncation of SutA-NTD (SutA (ΔN)) abolishes the activation activity of SutA. The data were presented as mean±S.E.M., n = 4. The individual data points were shown in black dots. Two-sided Student’s t test was performed. *p < 0.05, **p < 0.01, ***p < 0.001, n = 4. Source data are provided as a Source Data file.
