Fig. 3: The cryo-EM structure of Pae SutA-RNAP-σS complex.
From: Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding
a The cryo-EM map for SutA-RNAP-σS complex (open lobe). RNAP subunits are colored as the color scheme. SutA is colored in purple. The insert shows the map signal of the wedge loop of SutA in the RNAP-β protrusion/lobe gap. b The structural model of SutA-RNAP-σS complex (open lobe). The insert shows the surface presentation of the structure highlighting SutA wedge loop (SutA-WL) in RNAP-β protrusion/lobe gap and the open main cleft. c The cryo-EM map for SutA-RNAP-σS complex (closed lobe). The insert shows no signal of the wedge loop of SutA in RNAP-β protrusion/lobe gap. d The structural model for SutA-RNAP-σS complex (open lobe). The insert shows the surface presentation of the structure highlighting the disordered SutA-WL and the closed main cleft. e The interaction between SutA-RBD and RNAP-β protrusion. f The cryo-EM map for the middle helix of SutA-RBD. g The proposed route of the D/E-rich NTD (dashed purple line) entering the positively charged main cleft of RNAP. The electrostatic potential surface of RNAP was generated using APBS tools in Pymol.
