Fig. 2: Comparison of DiffScan and existing SVR detection methods in simulated datasets.

Default search length of 5 nt is used for deltaSHAPE and minimum search length of 5 nt is used for dStruct. The empirical model in Sükösd et al.40 was used to simulate reactivities. a Jaccard index between the top predicted nucleotides and the true SVRs at varying cutoffs. b Average distance between the top predicted nucleotides and the true SVRs at varying cutoffs. c Precision-Recall curves. Columns: three levels of strength of differential signals at simulated SVRs. Note deltaSHAPE does not allow external thresholding, and therefore it is represented as dots instead of curves.