Fig. 1: Fate mapping of CD201^hi Sca-1^hi HSCs.

a Subdivision of the immuno-phenotypic HSC population (LSK CD48^−/loCD150⁺) by CD201 (EPCR) and Sca-1 expression; ES-HSC, CD201 (EPCR)^hi Sca-1^hi HSCs. Relations between HSC populations in native hematopoiesis and how they connect to lineage pathways will be studied. b Bone marrow populations of Fgd5^{ZsGreen:CreERT2}/R26^LSL-tdRFP mice were analyzed 10–11 days after TAM induction for RFP expression (n = 20, 4 independent experiments, means and individual values are shown; one-way ANOVA with Sidak correction; ns, not significant; p values shown in graph); for flow cytometry phenotypes see supplementary Fig. 1a. c–e To map HSC fate over time, Fgd5^{ZsGreen:CreERT2}/R26^LSL-tdRFP mice (n = 18, 10, 12, 10, 10, 8, and 12 mice/consecutive timepoint (1.5–92 weeks of chase)) were TAM-induced, groups of mice were sacrificed at indicated time points, and bone marrow populations and peripheral blood leukocytes were analyzed for RFP expression (pooled data from five independent experiments are shown). d Percentage of RFP⁺ HSCs and ES HSCs (mean and SEM). RFP-labeling of ES HSCs did not significantly rise (two-sided F-test on linear regression), while labeling of total HSCs did significantly increase during the catch-up phase (two-sided paired t-test, p-values for time points 1, 2, and 3: <0.001, 0.0057, and 0.0034). e Fractions of labeled peripheral blood and bone marrow cells relative to ES HSCs at indicated time points; the labeling frequencies of the cell populations from each individual were normalized to its ES HSC labeling at final bone marrow analysis. Means are shown. Source data for all main and supplementary figures are provided as a Source Data file. For transcriptome data, refer to “Data availability” section.